Temporary preparation of Stomata (on the ruled page/ right hand side) Aim: To prepare a temporary slide and observe stomata under a compound microscope. Materials required: Watch glass, dropper, forceps, stain- safranin, glycerine, brush, needle, petri dish, glass slide, cover slip, compound microscope. Procedure: Fold the leaf and carefully tear along the bruised area of the lower side of the leaf. We can see a colourless narrow border along the torn edge. Carefully pull out the thin membranous transparent layer from the lower epidermis using a forceps. Put the epidermis into a watch glass containing distilled water. Take few drops of Safranin solution using a dropper and transfer this into another watch glass. Using a brush transfer the epidermis into the watch glass containing the Safranin solution. Keep the epidermis for 30 sec in the Safranin solution to stain the peel. To remove excess stain sticking to the peel, place it again in the watch glass containin...